raman microspectroscopy of individual algal cells sensing unsaturation of storage lipids in vivo拉曼显微镜的藻细胞传感未饱和存储体内脂质.pdfVIP
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raman microspectroscopy of individual algal cells sensing unsaturation of storage lipids in vivo拉曼显微镜的藻细胞传感未饱和存储体内脂质
Sensors 2010, 10, 8635-8651; doi:10.3390/s100908635
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Raman Microspectroscopy of Individual Algal Cells: Sensing
Unsaturation of Storage Lipids in vivo
Ota Samek 1,*, Alexandr Jonáš 1, Zdeněk Pilát 1, Pavel Zemánek 1, Ladislav Nedbal 2,
Jan Tříska 2, Petr Kotas 2 and Martin Trtílek 3
1
Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech
Republic, Kralovopolska 147, 61264 Brno, Czech Republic; E-Mails: sasa@isibrno.cz (A.J.);
pilat@isibrno.cz (Z.P.); pavlik@isibrno.cz (P.Z.)
2
Institute of Systems Biology and Ecology of the AS CR, v.v.i., Academy of Sciences of the Czech
Republic, Zámek 136, 37333 Nové Hrady, Czech Republic; E-Mails: nedbal@greentech.cz (L.N.);
triska@usbe.cas.cz (J.T.); kotas@usbe.cas.cz (P.K.)
3 Photon Systems Instruments, Drásov 470, 664 24 Drásov, Czech Republic; E-Mail: martin@psi.cz
* Author to whom correspondence should be addressed; E-Mail: osamek@isibrno.cz;
Tel.: +420-541-514-127; Fax: +420-541-514-402.
Received: 30 July 2010; in revised form: 25 August 2010 / Accepted: 14 September 2010 /
Published: 17 September 2010
Abstract: Algae are becoming a strategic source of fuels, food, feedstocks, and
biologically active compounds. This potential has stimulated the development of
innovative analytical methods focused on these microorganisms. Algal lipids are among
the most promising potential products fo
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