regulation of the spontaneous augmentation of nav1.9 in mouse dorsal root ganglion neurons effect of pka and pkc pathways监管的自发增加nav1.9鼠背根神经节神经元pka和pkc途径的影响.pdfVIP

Mar. Drugs 2010, 8, 728-740; doi:10.3390/md8030728 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Regulation of the Spontaneous Augmentation of NaV 1.9 in Mouse Dorsal Root Ganglion Neurons: Effect of PKA and PKC Pathways Jun-ichi Kakimura 1,2,*, Taixing Zheng 2, Noriko Uryu 2 and Nobukuni Ogata 2 1 Technical Center, Hiroshima University, Hiroshima, 734-8551, Japan 2 Department of Neurophysiology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 734-8551, Japan; E-Mails: tei0322@hiroshima-u.ac.jp (T.Z.); uryu@asahi.ac.jp (N.U.); ogatan@hiroshima-u.ac.jp (N.O.) * Author to whom correspondence should be addressed; E-Mail: kakimura@hiroshima-u.ac.jp; Tel.: +81-82-257-5127; Fax: +81-82-257-5127. Received: 15 January 2010; in revised form: 8 February 2010 / Accepted: 18 March 2010 / Published: 19 March 2010 Abstract: Sensory neurons in the dorsal root ganglion express two kinds of tetrodotoxin resistant (TTX-R) isoforms of voltage-gated sodium channels, Na 1.8 and Na 1.9. These V V isoforms play key roles in the pathophysiology of chronic pain. Of special interest is NaV 1.9: our previous studies revealed a unique property of the NaV 1.9 current, i.e., the NaV 1.9 current shows a gradual and notable up-regulation of the peak amplitude during recording (“spontaneous augmentation of NaV 1.9”). However, the mechanism underlying the s