regulatory design governing progression of population growth phases in bacteria设计管理在细菌中人口增长的发展阶段.pdfVIP

Regulatory Design Governing Progression of Population Growth Phases in Bacteria ´ 1 . 2. 3 3 Agustino Martınez-Antonio * , Jason G. Lomnitz , Santiago Sandoval , Maximino Aldana , Michael A. Savageau2 ´ ´ ´ 1 Departamento de Ingenierıa Genetica, Cinvestav, Irapuato, Guanajuato, Mexico, 2 Biomedical Engineering Department, University of California Davis, Davis, California, ´ ´ ´ ´ United States of America, 3 Instituto de Fısica, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico Abstract It has long been noted that batch cultures inoculated with resting bacteria exhibit a progression of growth phases traditionally labeled lag, exponential, pre-stationary and stationary. However, a detailed molecular description of the mechanisms controlling the transitions between these phases is lacking. A core circuit, formed by a subset of regulatory interactions involving five global transcription factors (FIS, HNS, IHF, RpoS and GadX), has been identified by correlating information from the well- established transcriptional regulatory network of Escherichia coli and genome-wide expression data from cultures in these different growth phases. We propose a functional role for this circuit in controlling progression through these phases. Two alternative hypotheses for controlling the transition between the growth phases are first, a continuous graded adjustment to changing environmental conditions, and second, a discontinuous hysteretic switch at critical thr