regulatory feedback loop of two phz gene clusters through 5′-untranslated regions in pseudomonas sp. m18监管反馈回路的两个phz基因簇到5u2032非翻译地区假单胞菌m18 sp.pdfVIP

Regulatory Feedback Loop of Two phz Gene Clusters through 59-Untranslated Regions in Pseudomonas sp. M18 1,3 1 2 1 1 1 1 Yaqian Li , Xilin Du , Zhi John Lu , Daqiang Wu , Yilei Zhao , Bin Ren , Jiaofang Huang , Xianqing 1 3 1 Huang , Yuhong Xu , Yuquan Xu * 1 State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China, 2 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America, 3 School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China Abstract Background: Phenazines are important compounds produced by pseudomonads and other bacteria. Two phz gene clusters called phzA1-G1 and phzA2-G2, respectively, were found in the genome of Pseudomonas sp. M18, an effective biocontrol agent, which is highly homologous to the opportunistic human pathogen P. aeruginosa PAO1, however little is known about the correlation between the expressions of two phz gene clusters. Methodology/Principal Findings: Two chromosomal insertion inactivated mutants for the two gene clusters were constructed respectively and the correlation between the expressions of two phz gene clusters was investigated in strain M18. Phenazine-1-carboxylic acid (PCA) molecules produced from phzA2-G2 gene cluster are able to auto-regulate expression itself and activate the expression of phzA1-G1 gene cluster in a circulated amplification pattern. However, the post-transcriptional expression of phzA1-G1 transcript was blocked principally through 5 9-untranslated region (UTR). In contrast, the phzA2-G2