review of transducer principles for label-free biomolecular interaction analysis回顾label-free传感器原理的生物分子相互作用分析.pdf

Biosensors 2011, 1, 70-92; doi:10.3390/bios 1030070 OPEN ACCESS biosensors ISSN 2079-6374 /journal/biosensors/ Review Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis Martin Nirschl 1,*, Florian Reuter 2 and Janos Vörös 1 1 Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland; E-Mail: janos.voros@biomed.ee.ethz.ch 2 Siemens Technology Accelerator GmbH, Otto-Hahn-Ring 6, 81739 Munich, Germany; E-Mail: florian.reuter@ * Author to whom correspondence should be addressed; E-Mail: nirschlm@ethz.ch; Tel.: +49-163-698-6859. Received: 19 May 2011; in revised form: 18 June 2011 / Accepted: 29 June 2011 / Published: 1 July 2011 Abstract: Label-free biomolecular interaction analysis is an important technique to study the chemical binding between e.g., protein and protein or protein and small molecule in real-time. The parameters obtained with this technique, such as the affinity, are important for drug development. While the surface plasmon resonance (SPR) instruments are most widely used, new types of sensors are emerging. These developments are generally driven by the need for higher throughput, lower sample consumption or by the need of complimentary information to the SPR data. This review aims to give an overview about a wide range of sensor transducers, the working principles and the peculiarities of each technology, e.g., concerning the set-up, sensitivity, sensor si