rhinovirus genome evolution during experimental human infection在实验人类感染鼻病毒基因组的进化.pdfVIP

Rhinovirus Genome Evolution during Experimental Human Infection 1,2 . 3,4. 3,4 1,2 5 Samuel Cordey * , Thomas Junier , Daniel Gerlach , Francesca Gobbini , Laurent Farinelli , 3,4 6 1,2. 1,2. Evgeny M. Zdobnov , Birgit Winther , Caroline Tapparel , Laurent Kaiser 1 Laboratory of Virology, Division of Infectious Diseases and Division of Laboratory Medicine, University of Geneva Hospitals, Geneva, Switzerland, 2 Medical School, University of Geneva, Geneva, Switzerland, 3 Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland, 4 Swiss Institute of Bioinformatics, Geneva, Switzerland, 5 Fasteris SA, Plan-les-Ouates, Switzerland, 6 Division of General Pediatrics, Department of Pediatrics, University of Virginia, Charlottesville, Virginia, United States of America Abstract Human rhinoviruses (HRVs) evolve rapidly due in part to their error-prone RNA polymerase. Knowledge of the diversity of HRV populations emerging during the course of a natural infection is essential and represents a basis for the design of future potential vaccines and antiviral drugs. To evaluate HRV evolution in humans, nasal wash samples were collected daily for five days from 15 immunocompetent volunteers experimentally infected with a reference stock of HRV-39. In parallel, HeLa-OH cells were inoculated to compare HRV evolution in vitro. Nasal wash in vivo assessed by real-time PCR showed a viral load that peaked at 48–72 h. Ultra-deep sequencing was used to compare the low-freque