rheotaxis in larval zebrafish is mediated by lateral line mechanosensory hair cells在斑马鱼窃是由侧线mechanosensory毛细胞.pdfVIP

Rheotaxis in Larval Zebrafish Is Mediated by Lateral Line Mechanosensory Hair Cells 1 2 3 1,3 Arminda Suli , Glen M. Watson , Edwin W. Rubel , David W. Raible * 1 Department of Biological Structure, University of Washington, Seattle, Washington, United States of America, 2 Department of Biology, University of Louisiana Lafayette, Lafayette, Louisiana, United States of America, 3 V. M. Bloedel Hearing Research Center, University of Washington, Seattle, Washington, United States of America Abstract The lateral line sensory system, found in fish and amphibians, is used in prey detection, predator avoidance and schooling behavior. This system includes cell clusters, called superficial neuromasts, located on the surface of head and trunk of developing larvae. Mechanosensory hair cells in the center of each neuromast respond to disturbances in the water and convey information to the brain via the lateral line ganglia. The convenient location of mechanosensory hair cells on the body surface has made the lateral line a valuable system in which to study hair cell damage and regeneration. One way to measure hair cell survival and recovery is to assay behaviors that depend on their function. We built a system in which orientation against constant water flow, positive rheotaxis, can be quantitatively assessed. We found that zebrafish larvae perform positive rheotaxis and that, similar to adult fish, larvae use both visual and lateral line input to perform this behavior. Disruption or damage of hair cells in the absence of vision leads to a marked decrease in rheotaxis that recovers upon hair cell repair or regeneration. Citation: Suli A, Watson GM, Rubel EW, Raible DW (2012) Rheotaxis in Larval Zebrafish Is Mediated by L