role of different pre-treatments on composition and rheology of synovial fluids角色不同的预处理滑膜液的成分和流变学.pdfVIP

Polymers 2009, 1, 16-34; doi:10.3390/polym 1010016 OPEN ACCESS polymers ISSN 2073-4360 /journal/polymers Article Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids Marguerite Rinaudo 1,2,*, Yves Rozand 3, Pierre Mathieu 4 and Thierry Conrozier 5 1 Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP53, 38041 Grenoble cedex 9, France 2 Joseph Fourier University, BP53, 38041 Grenoble cedex 9, France 3 Centre de Rhumatologie, 25, boulevard Agutte Sembat, 38000 Grenoble, France; E-Mail: yvesrozand@orange.fr 4 Laboratoire d’exploration et de morphométrie articulaire, Centre hospitalier Lyon –Sud, Pierre-Bénite, France; E-Mail: pierre.mathieu42@wanadoo.fr 5 Service de Rhumatologie, Centre hospitalier Lyon –Sud, Pierre-Bénite, France; E-Mail: thierry.conrozier@wanadoo.fr * Author to whom correspondence should be addressed; E-Mail: marguerite.rinaudo@rs.fr; Tel.: +33-476037627; Fax: +33-476547203. Received: 28 October 2009; in revised form: 16 December 2009 / Accepted: 28 December 2009 / Published: 28 December 2009 Abstract: Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight dis