role of trehalose in salinity and temperature tolerance in the model halophilic bacterium chromohalobacter salexigens海藻糖在盐度和温度公差模型中的嗜盐细菌chromohalobacter salexigens.pdfVIP

Role of Trehalose in Salinity and Temperature Tolerance in the Model Halophilic Bacterium Chromohalobacter salexigens 1. ˜ 1. 1 ´ 1 Mercedes Reina-Bueno , Montserrat Argandona , Manuel Salvador , Javier Rodrıguez-Moya , 2 3 ´ 1 1 Fernando Iglesias-Guerra , Laszlo N. Csonka , Joaquın J. Nieto , Carmen Vargas * 1 Department of Microbiology and Parasitology, University of Seville, Seville, Spain, 2 Department of Organic and Pharmaceutical Chemistry, University of Seville, Seville, Spain, 3 Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America Abstract The disaccharide trehalose is considered as a universal stress molecule, protecting cells and biomolecules from injuries imposed by high osmolarity, heat, oxidation, desiccation and freezing. Chromohalobacter salexigens is a halophilic and extremely halotolerant c-proteobacterium of the family Halomonadaceae. In this work, we have investigated the role of trehalose as a protectant against salinity, temperature and desiccation in C. salexigens. A mutant deficient in the trehalose-6- phosphate synthase gene (otsA::V) was not affected in its salt or heat tolerance, but double mutants ectoine- and trehalose- deficient, or hydroxyectoine-reduced and trehalose-deficient, displayed an osmo- and thermosensitive phenotype, respectively. This suggests a role of trehalose as a secondary solute i