rop gtpase-dependent actin microfilaments promote pin1 polarization by localized inhibition of clathrin-dependent endocytosis罗普gtpase-dependent促进肌动蛋白微丝骨架pin1极化的本地化抑制clathrin-dependent内吞作用.pdfVIP

ROP GTPase-Dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin- Dependent Endocytosis 1. 1,2. 1,3 4 3 5 Shingo Nagawa , Tongda Xu , Deshu Lin , Pankaj Dhonukshe , Xingxing Zhang , Jiri Friml , 4 3 1 Ben Scheres , Ying Fu , Zhenbiao Yang * 1 Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, California, United States of America, 2 Temasek Lifesciences Laboratory Ltd, National University of Singapore, Singapore, 3 State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing, China, 4 Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands, 5 Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Ghent, Belgium Abstract Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetri