s phase entry of neural progenitor cells correlates with increased blood flow in the young subventricular zone进入s期的神经祖细胞与血流量增加的年轻subventricular区.pdfVIP

S Phase Entry of Neural Progenitor Cells Correlates with Increased Blood Flow in the Young Subventricular Zone 1.¤ 2,4,5. 1 2,3,4,5 Benjamin Lacar , Peter Herman , Nathaniel W. Hartman , Fahmeed Hyder , Angelique Bordey1* 1 Department of Neurosurgery and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, United States of America, 2 Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, United States of America, 3 Department of Biomedical Engineering, Yale University School of Medicine, New Haven, Connecticut, United States of America, 4 Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut, United States of America, 5 Core Center for Quantitative Neuroscience with Magnetic Resonance, Yale University School of Medicine, New Haven, Connecticut, United States of America Abstract The postnatal subventricular zone (SVZ) contains proliferating neural progenitor cells in close proximity to blood vessels. Insults and drug treatments acutely stimulate cell proliferation in the SVZ, which was assessed by labeling cells entering S phase. Although G1-to-S progression is metabolically demanding on a minute-to-hour time scale, it remains unknown whether increased SVZ cell proliferation is accompanied by a local hemodynamic response. This neurovascular coupling provides energy substrates to active neuronal assemblies. Transcardial dye perfusion revealed the presence of capillaries throughout the SVZ that constrict upon applications of the thromboxane A2 receptor agonist U-46119 in acute brain slice preparations. We then monitored in vivo blood flow using laser Doppler flowmetry via a microprobe