原创力文档screening of new microsatellite dna markers from the genome of platyeriocheir formosa筛选新从platyeriocheir福尔摩沙的基因组微卫星dna标记.pdfVIP
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screening of new microsatellite dna markers from the genome of platyeriocheir formosa筛选新从platyeriocheir福尔摩沙的基因组微卫星dna标记
Int. J. Mol. Sci. 2012, 13, 5598-5606; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Screening of New Microsatellite DNA Markers from the
Genome of Platyeriocheir formosa
Hui-Ling Cheng 1, Yan-Horn Lee 2, Dai-Shion Hsiung 3 and Mei-Chen Tseng 3,*
1 Department of Environmental Resources Management, Chia Nan University of Pharmacy and Science,
Tainan 717, Taiwan; E-Mail: lynn62359@.tw
2 Biotechnology Division, Fisheries Research Institute Council of Agriculture, Tungkang,
Pingtung 928, Taiwan; E-Mail: leeyh4331@
3 Department of Aquaculture, National Pingtung University of Science and Technology,
Pingtung 912, Taiwan; E-Mail: bear000000@.tw
* Author to whom correspondence should be addressed; E-Mail: mctseng@.tw;
Tel.: +886-8-7703202 (ext. 6227); Fax: +886-8-7740401.
Received: 20 February 2012; in revised form: 22 April 2012 / Accepted: 24 April 2012 /
Published: 9 May 2012
Abstract: The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To
conserve P. formosa population diversity and ensure the sustainable use of this natural
resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to
promote the study of its population genetics in the future. In this study, more than
70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the
genetic diversity of P. formos
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