selective reduction of post-selection cd8 thymocyte proliferation in il-15rα deficient mice的选择性还原后选择cd8 il-15rα缺陷小鼠胸腺细胞增殖.pdfVIP

Selective Reduction of Post-Selection CD8 Thymocyte Proliferation in IL-15Ra Deficient Mice 1,2. 2,3,4. 5. 3 2 Kai-Ping N. Chow , Jian-Tai Qiu , Jam-Mou Lee , Shuo-Lun Hsu , Shan-Che Yang , Ning-Ning 2 3 2,3 Wu , Wei Huang , Tzong-Shoon Wu * 1 Department of Microbiology and Immunology, School of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China, 2 Graduate Institute of Biomedical Sciences, School of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China, 3 Department of Biomedical Sciences, School of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China, 4 Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, Republic of China, 5 Wellkent Biomedical Technology Inc., Taipei, Taiwan, Republic of China Abstract Peripheral CD8+ T cells are defective in both IL-15 and IL-15Ra knock-out (KO) mice; however, whether IL-15/IL-15Ra deficiency has a similar effect on CD8 single-positive (SP) thymocytes remains unclear. In this study, we investigated whether the absence of IL-15 transpresentation in IL-15Ra KO mice results in a defect in thymic CD8 single positive (SP) TCRhi thymocytes. Comparison of CD8SP TCRhi thymocytes from IL-15Ra KO mice with their wild type (WT) counterparts by flow cytometry showed a significant reduction in the percentage of CD692 CD8SP TCRhi thymocytes, which represent thymic premigrants. In addition, analysis of in vivo 5-bromo-2-deoxyuridine (BrdU) incorporation demonstrated that premigrant expansion of CD8SP TCRhi thymo