selenium derivatization of nucleic acids for phase and structure determination in nucleic acid x-ray crystallography硒衍生化阶段和结构测定核酸的核酸x射线晶体学.pdfVIP

Int. J. Mol. Sci. 2008, 9, 258-271 International Journal of Molecular Sciences ISSN 1422-0067 © 2008 by MDPI /ijms Review Selenium Derivatization of Nucleic Acids for Phase and Structure Determination in Nucleic Acid X-ray Crystallography Jia Sheng and Zhen Huang * Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA * Author to whom correspondence should be addressed. E-mail: huang@ Received: 3 December 2007; in revised form: 18 February 2008 / Accepted: 25 February 2008 / Published: 12 March 2008 Abstract: Selenium derivatization (via selenomethionine) of proteins for crystal structure determination via MAD phasing has revolutionized protein X-ray crystallography. It is estimated that over two thirds of all new crystal structures of proteins have been determined via Se-Met derivatization. Similarly, selenium functionalities have also been successfully incorporated into nucleic acids to facilitate their structure studies and it has been proved that this Se-derivatization has advantages over halogen strategy, which was usually used as a traditional method in this field. This review reports the development of site-specific selenium derivatization of nucleic acids for phase determination since the year of 2001 (mainly focus on the 2’-position of the ribose). All the synthesis of 2’-SeMe