self-assembling peptide nanofiber scaffolds accelerate wound healing自组装多肽纳米纤维支架加速伤口愈合.pdfVIP

Self-Assembling Peptide Nanofiber Scaffolds Accelerate Wound Healing Aurore Schneider1,3, Jonathan A. Garlick1,2, Christophe Egles1,2* 1 Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, Tufts University, School of Dental Medicine, Boston, Massachusetts, United States of America, 2 Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, United States of America, 3 Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America Cutaneous wound repair regenerates skin integrity, but a chronic failure to heal results in compromised tissue function and increased morbidity. To address this, we have used an integrated approach, using nanobiotechnology to augment the rate of wound reepithelialization by combining self-assembling peptide (SAP) nanofiber scaffold and Epidermal Growth Factor (EGF). This SAP bioscaffold was tested in a bioengineered Human Skin Equivalent (HSE) tissue model that enabled wound reepithelialization to be monitored in a tissue that recapitulates molecular and cellular mechanisms of repair known to occur in human skin. We found that SAP underwent molecular self-assembly to form unique 3D structures that stably covered the surface of the wound, suggesting that this scaffold may serve as a viable wound dressing. We measured the rates of release of EGF from the SAP scaffold and determined that EGF was only released when the scaffold was in direct contact with the HSE. By measuring the length of the epithelial tongue during wound reepithelialization, we found that SAP scaffolds containing EGF accelerated the rate of wound coverage by 5 fold when compared to controls without scaffolds and by 3.5 fold when compared to the scaffold without EGF. In conclusion, our experiments demonstrated that biomaterials composed of a biofunctionalized pe