sol-gel entrapped candida antarctica lipase b — a biocatalyst with excellent stability for kinetic resolution of secondary alcohols溶胶-凝胶包埋南极假丝酵母脂肪酶b - a生物催化剂的稳定性动力学解决二级醇.pdfVIP

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sol-gel entrapped candida antarctica lipase b — a biocatalyst with excellent stability for kinetic resolution of secondary alcohols溶胶-凝胶包埋南极假丝酵母脂肪酶b - a生物催化剂的稳定性动力学解决二级醇.pdf

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sol-gel entrapped candida antarctica lipase b — a biocatalyst with excellent stability for kinetic resolution of secondary alcohols溶胶-凝胶包埋南极假丝酵母脂肪酶b - a生物催化剂的稳定性动力学解决二级醇

Molecules 2012, 17, 13045-13061; doi:10.3390/molecules171113045 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols Anca Ursoiu 1, Cristina Paul 1, Tibor Kurtán 2 and Francisc Péter 1,* 1 Faculty of Industrial Chemistry and Environmental, University “Politehnica” of Timisoara, C. Telbisz 6, 300001 Timisoara, Romania; E-Mails: anca.ursoiu@chim.upt.ro (A.U.); cristina.paul@chim.upt.ro (C.P.) 2 Department of Organic Chemistry, University of Debrecen, Debrecen 4032, Egyetem tér 1, Hungary; E-Mail: kurtan.tibor@science.unideb.hu * Author to whom correspondence should be addressed; E-Mail: francisc.peter@chim.upt.ro; Tel.: +40-256-404-216; Fax: +40-256-403-060. Received: 26 September 2012; in revised form: 15 October 2012 / Accepted: 30 October 2012 / Published: 2 November 2012 Abstract: Sol-gel entrapment is an efficient immobilization technique that allows preparation of robust and highly stable biocatalysts. Lipase from Candida antarctica B was immobilized by sol-gel entrapment and by sol-gel entrapment combined with adsorption on Celite 545, using a ternary silane precursor system. After optimization of the immobilization protocol, the best enzyme loading was 17.4 mg/g support for sol-gel entrapped lipase and 10.7 mg/g support for samples obtained by entrapment and adsorption. Sol-gel immobilized enzymes