subdominant antigens in bacterial vaccines am779 is subdominant in the anaplasma marginale outer membrane vaccine but does not associate with protective immunity下属音抗原在红孢子虫属的细菌疫苗am779下属音边际外膜疫苗但不与保护性免疫.pdfVIP

Subdominant Antigens in Bacterial Vaccines: AM779 Is Subdominant in the Anaplasma marginale Outer Membrane Vaccine but Does Not Associate with Protective Immunity 1 2 3 1,2 3 2 Saleh M. Albarrak , Wendy C. Brown , Susan M. Noh , Kathryn E. Reif , Glen A. Scoles , Joshua E. Turse , 2 3 1,2 Junzo Norimine , Massaro W. Ueti , Guy H. Palmer * 1 Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, United States of America, 2 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America, 3 Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, Washington, United States of America Abstract Identification of specific antigens responsible for the ability of complex immunogens to induce protection is a major goal in development of bacterial vaccines. Much of the investigation has focused on highly abundant and highly immunodominant outer membrane proteins. Recently however, genomic and proteomic approaches have facilitated identification of minor components of the bacterial outer membrane that have previously been missed or ignored in immunological analyses. Immunization with Anaplasma marginale outer membranes or a cross-linked surface complex induces protection against bacteremia, however the components responsible for protection within these complex immunogens are unknown. Using outer membrane protein AM779 as a model, we demonstrated that this highly conserved but minor component of the A. marginale surface was immunologically sub-dominant