synergism and rules from combination of baicalin, jasminoidin and desoxycholic acid in refined qing kai ling for treat ischemic stroke mice model合作和规则从黄芩苷、栀子苷和desoxycholic酸精制清开灵治疗缺血性中风小鼠模型.pdfVIP

Synergism and Rules from Combination of Baicalin, Jasminoidin and Desoxycholic acid in Refined Qing Kai Ling for Treat Ischemic Stroke Mice Model 1. 2. 3. 4 2 4 Jian Li , Run-guo Wu , Fan-yun Meng , Zhong Wang , Chang-ming Wang , Yong-yan Wang , Zhan- jun Zhang2* 1 School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing, People’s Republic of China, 2 State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China, 3 School of Resources Science Technology, Beijing Normal University, Beijing, China, 4 Institute of Basic Research in Clinical Medicine, China Academy of Traditional Chinese Medicine, Beijing, China Abstract Refined Qing-Kai-Ling (QKL), a modified Chinese medicine, consists of three main ingredients (Baicalin, Jasminoidin and Desoxycholic acid), plays a synergistic effect on the treatment of the acute stage of ischemic stroke. However, the rules of the combination and synergism are still unknown. Based on the ischemic stroke mice model, all different kinds of combination of Baicalin, Jasminoidin, and Desoxycholic acid were investigated by the methods of neurological examination, microarray, and genomics analysis. As a result, it confirmed that the combination of three drugs offered a better therapeutical effect on ischemic stroke than monotherapy of each drug. Additionally, we used Ingenuity pathway Analysis (IPA) and principal component analysis (PCA) to extract the dominant information of expression changes in 373 ischemia- related genes. The results suggested that 5 principal components (PC1-5) could account for more than 95% energy in the gene