systems biology meets stress ecology linking molecular and organismal stress responses in daphnia magna系统生物学与压力连接分子生态学和生物的应激反应在水蚤麦格纳.pdfVIP

Heckmann et al. Genome Biology 2008, 9:R40 /2008/9/2/R40 Daphnia Genomics Consortium RESEARCH Open Access Systems biology meets stress ecology: linking molecular and organismal stress responses in Daphnia magna 1,2* 1 1,3 1 4,5 Lars-Henrik Heckmann , Richard M Sibly , Richard Connon , Helen L Hooper , Thomas H Hutchinson , Steve J Maund6 1 1 1 , Christopher J Hill , Anthony Bouetard , Amanda Callaghan Abstract Background: Ibuprofen and other nonsteroidal anti-inflammatory drugs have been designed to interrupt eicosanoid metabolism in mammals, but little is known of how they affect nontarget organisms. Here we report a systems biology study that simultaneously describes the transcriptomic and phenotypic stress responses of the model crustacean Daphnia magna after exposure to ibuprofen. Results: Our findings reveal intriguing similarities in the mode of action of ibuprofen between vertebrates and invertebrates, and they suggest that ibuprofen has a targeted impact on reproduction at the molecular, organismal, and population level in daphnids. Microarray expression and temporal real-time quantitative PCR profiles of key genes suggest early ibuprofen interruption of crustacean eicosanoid metabolism, which appears to disrupt signal transduction affecting juvenile hormone metabolism and oogenesis. Conclusion: Combining molecular and organismal stress responses provides a guide to possible chronic consequences of environmental stress for population health. This could improve current environmental risk assessment by providing an ear