terpenes as green solvents for extraction of oil from microalgae萜烯从微藻作为绿色溶剂提取石油.pdfVIP

Molecules 2012, 17, 8196-8205; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Terpenes as Green Solvents for Extraction of Oil from Microalgae Celine Dejoye Tanzi, Maryline Abert Vian *, Christian Ginies, Mohamed Elmaataoui and Farid Chemat Université d’Avignon et des Pays du Vaucluse, INRA, UMR408, UMR406, 84000 Avignon, France; E-Mails: celine.dejoye@univ-avignon.fr (C.D.T.); christian.ginies@avignon.inra.fr (C.G.); mohamed.elmaataoui@univ-avignon.fr (M.E.); farid.chemat@univ-avignon.fr (F.C.) * Author to whom correspondence should be addressed; E-Mail: maryline.vian@univ-avignon.fr; Tel.: +33-490-144-432; Fax: +33-490-144-441. Received: 1 June 2012; in revised form: 21 June 2012 / Accepted: 26 June 2012 / Published: 9 July 2012 Abstract: Herein is described a green and original alternative procedure for the extraction of oil from microalgae. Extractions were carried out using terpenes obtained from renewable feedstocks as alternative solvents instead of hazardous petroleum solvents such as n-hexane. The described method is achieved in two steps using Soxhlet extraction followed by the elimination of the solvent from the medium using Clevenger distillation in the second step. Oils extracted from microalgae were compared in terms of qualitative and quantitative determination. No significant difference was obtained between each extract, allowing us to conclude that the proposed method is green, clean and efficient. Keywords: lipid e