th1-th17 cells mediate protective adaptive immunity against staphylococcus aureus and candida albicans infection in miceth1-th17细胞调节适应性免疫保护对金黄色葡萄球菌和白色念珠菌感染的老鼠.pdfVIP

Th1-Th17 Cells Mediate Protective Adaptive Immunity against Staphylococcus aureus and Candida albicans Infection in Mice 1 1,2 3 3 1 1 Lin Lin , Ashraf S. Ibrahim , Xin Xu , Joshua M. Farber , Valentina Avanesian , Beverlie Baquir , Yue Fu1,2, Samuel W. French2,4, John E. Edwards Jr.1,2, Brad Spellberg1,2,5* 1The Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California at Los Angeles (UCLA) Medical Center, Torrance, California, United States of America, 2 The David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America, 3 Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 4 The Department of Pathology, Harbor-UCLA Medical Center, Torrance, California, United States of America, 5 The Division of General Internal Medicine, Harbor-UCLA Medical Center, Torrance, California, United States of America Abstract We sought to define protective mechanisms of immunity to Staphylococcus aureus and Candida albicans bloodstream infections in mice immunized with the recombinant N-terminus of Als3p (rAls3p-N) vaccine plus aluminum hydroxide (Al(OH3) adjuvant, or adjuvant controls. Deficiency of IFN-c but not IL-17A enhanced susceptibility of control mice to both infections. However, vaccine-induced protective immunity against both infections required CD4+ T-cell-derived IFN-c and IL-17A, and functional phagocytic effectors. Vaccination primed Th1, Th17, and Th1/17 lymphocytes, which produced pro- inflammatory cytokines that enhanced phagocytic killing of both organis