the acinetobacter baumannii enta gene located outside the acinetobactin cluster is critical for siderophore production, iron acquisition and virulence鲍曼不动杆菌enta基因位于acinetobactin集群外的含铁细胞生产至关重要,铁收购和毒性.pdfVIP

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2017-09-12 发布于上海
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the acinetobacter baumannii enta gene located outside the acinetobactin cluster is critical for siderophore production, iron acquisition and virulence鲍曼不动杆菌enta基因位于acinetobactin集群外的含铁细胞生产至关重要,铁收购和毒性.pdf

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the acinetobacter baumannii enta gene located outside the acinetobactin cluster is critical for siderophore production, iron acquisition and virulence鲍曼不动杆菌enta基因位于acinetobactin集群外的含铁细胞生产至关重要,铁收购和毒性

The Acinetobacter baumannii entA Gene Located Outside the Acinetobactin Cluster Is Critical for Siderophore Production, Iron Acquisition and Virulence William F. Penwell, Brock A. Arivett, Luis A. Actis* Department of Microbiology, Miami University, Oxford, Ohio, United States of America Abstract Acinetobacter baumannii causes severe infections in compromised patients, who present an iron-limited environment that controls bacterial growth. This pathogen has responded to this restriction by expressing high-affinity iron acquisition systems including that mediated by the siderophore acinetobactin. Gene cloning, functional assays and biochemical tests showed that the A. baumannii genome contains a single functional copy of an entA ortholog. This gene, which is essential for the biosynthesis of the acinetobactin precursor 2,3-dihydroxybenzoic acid (DHBA), locates outside of the acinetobactin gene cluster, which otherwise harbors all genes needed for acinetobactin biosynthesis, export and transport. In silico analyses and genetic complementation tests showed that entA locates next to an entB ortholog, which codes for a putative protein that contains the isochorismatase lyase domain, which is needed for DHBA biosynthesis from isochorismic acid, but lacks the aryl carrier protein domain, which is needed for tethering activated DHBA and completion of siderophore biosynthesis. Thus, basF, which locates within the acinetobactin gene cluster, is the only fully functional entB ortholog present in ATCC 19606T. The differences in amino acid length and sequences between these two EntB orthologs and the differences in the genetic context within which the entA and entB genes are found in different A. baumannii isolates indicate that they were acquired from different sources by horizontal transfer. Interestingly, the AYE strain proved to be a natural entA mut