the cell cycle–regulated genes of schizosaccharomyces pombe粟酒裂殖酵母的细胞cycle-regulated基因.pdfVIP

Open access, freely available online PLoS BIOLOGY The Cell Cycle–Regulated Genes of Schizosaccharomyces pombe 1 1 1 2 1 2 Anna Oliva , Adam Rosebrock , Francisco Ferrezuelo , Saumyadipta Pyne , Haiying Chen , Steve Skiena , 1* 1* Bruce Futcher , Janet Leatherwood 1 Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, United States of America, 2 Department of Computer Science, Stony Brook University, Stony Brook, New York, United States of America Many genes are regulated as an innate part of the eukaryotic cell cycle, and a complex transcriptional network helps enable the cyclic behavior of dividing cells. This transcriptional network has been studied in Saccharomyces cerevisiae (budding yeast) and elsewhere. To provide more perspective on these regulatory mechanisms, we have used microarrays to measure gene expression through the cell cycle of Schizosaccharomyces pombe (fission yeast). The 750 genes with the most significant oscillations were identified and analyzed. There were two broad waves of cell cycle transcription, one in early/mid G2 phase, and the other near the G2/M transition. The early/mid G2 wave included many genes involved in ribosome biogenesis, possibly explaining the cell cycle oscillation in protein synthesis in S. pombe. The G2/M wave included at least three distinctly regulated clusters of genes: one large cluster including mitosis, mitotic exit, and cell separation functions, one small cluster dedicated to DNA replication, and another small cluster dedicated to cytokinesis and division. S. pombe cell cycle