the chick embryo chorioallantoic membrane as an in vivo assay to study antiangiogenesis小鸡胚胎绒毛膜尿囊的膜作为体内试验研究抗血管.pdfVIP
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the chick embryo chorioallantoic membrane as an in vivo assay to study antiangiogenesis小鸡胚胎绒毛膜尿囊的膜作为体内试验研究抗血管
Pharmaceuticals 2010, 3, 482-513; doi:10.3390/ph3030482
OPEN ACCESS
pharmaceuticals
ISSN 1424-8247
/journal/pharmaceuticals
Review
The Chick Embryo Chorioallantoic Membrane as an In Vivo
Assay to Study Antiangiogenesis
Domenico Ribatti
Department of Human Anatomy and Histology, University of Bari Medical School, Piazza G. Cesare,
11, Policlinico 70124, Bari, Italy; E-Mail: ribatti@anatomia.uniba.it; Tel.: +39-080-547-8240; Fax:
+39-080-547-8310.
Received: 12 January 2010; in revised form: 29 January 2010 / Accepted: 2 March 2010 /
Published: 8 March 2010
Abstract: Antiangiogenesis, e.g., inhibition of blood vessel growth, is being investigated as
a way to prevent the growth of tumors and other angiogenesis-dependent diseases.
Pharmacological inhibition interferes with the angiogenic cascade or the immature
neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or
biological antagonists. The chick embryo chorioallantoic membrane (CAM) is an
extraembryonic membrane, which serves as a gas exchange surface and its function is
supported by a dense capillary network. Because its extensive vascularization and easy
accessibility, CAM has been used to study morphofunctional aspects of the angiogenesis
process in vivo and to study the efficacy and mechanism of action of pro- and
anti-angiogenic molecules. The fields of application of CAM in the stud
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