the 11s globulin sin a 2 from yellow mustard seeds shows ige cross-reactivity with homologous counterparts from tree nuts and peanut11 s球蛋白罪黄芥末种子2显示ige大与同源同行从树坚果和花生.pdfVIP

Sirvent et al. Clinical and Translational Allergy 2012, 2:23 /content/2/1/23 RESEARCH Open Access The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut 1 1 2 2 1 1 Sofía Sirvent , Martial Akotenou , Javier Cuesta-Herranz , Andrea Vereda , Rosalía Rodríguez , Mayte Villalba and Oscar Palomares1* Abstract Background: The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods: Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT), enzyme-linked immunosorbent assay (ELISA), immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results: The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut) but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conser