原创力文档the choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particlesresin-bound配体的选择影响的结构和免疫原性column-purified人类乳头瘤病毒16型病毒样颗粒.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
the choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particlesresin-bound配体的选择影响的结构和免疫原性column-purified人类乳头瘤病毒16型病毒样颗粒
The Choice of Resin-Bound Ligand Affects the Structure
and Immunogenicity of Column-Purified Human
Papillomavirus Type 16 Virus-Like Particles
Hyoung Jin Kim, Su Jeung Lim, Hye-Lim Kwag, Hong-Jin Kim*
College of Pharmacy, Chung-Ang University, Seoul, South Korea
Abstract
Cell growth conditions and purification methods are important in determining biopharmaceutical activity. However, in
studies aimed at manufacturing virus-like particles (VLPs) for the purpose of creating a prophylactic vaccine and antigen for
human papillomavirus (HPV), the effects of the presence of a resin-bound ligand during purification have never been
investigated. In this study, we compared the structural integrity and immunogenicity of two kinds of VLPs derived from HPV
type 16 (HPV16 VLPs): one VLP was purified by heparin chromatography (hHPV16 VLP) and the other by cation-exchange
chromatography (cHPV16 VLP). The reactivity of anti-HPV16 neutralizing monoclonal antibodies (H16.V5 and H16.E70)
towards hHPV16 VLP were significantly higher than the observed cHPV16 VLP reactivities, implying that hHPV16 VLP
possesses a greater number of neutralizing epitopes and has a greater potential to elicit anti-HPV16 neutralizing antibodies.
After the application of heparin chromatography, HPV16 VLP has a higher affinity for H16.V5 and H16.E70. This result
indicates that heparin chromatography is valuable in selecting functional HPV16 VLPs. In regard to VLP immunogenicity, the
anti-HPV16 L1 IgG and neutralizing antibody levels elicited by immunizations of mice with hHPV16 VLPs were higher than
those elicited by cHPV16 VLP with and without adjuvant. Therefore, the ability of hHPV16 VLP to elicit humoral immune
responses was superior to that of cHPV16 VLP. We conclude that the specific chromatographic technique employed for the
purification
您可能关注的文档
- the 5′utr-intron of the gladiolus polyubiquitin promoter gubq1 enhances translation efficiency in gladiolus and arabidopsis的5u2032utr-intron剑兰polyubiquitin发起人gubq1提高翻译效率在剑兰和拟南芥.pdf
- the 32nd annual congress of the society of critical care medicine, 28 january – 2 february 2003, san antonio, usa第32届国会危重病医学协会的,1月28日- 2 2003年2月,美国圣安东尼奥.pdf
- the 1000 genomes project new opportunities for research and social challenges1000人基因组计划研究新的机遇和社会挑战.pdf
- the 21st international symposium on intensive care and emergency medicine, brussels, belgium, 20-23 march 200121重症监护、急诊医学国际研讨会布鲁塞尔,比利时,2001年3月20 - 23日.pdf
- the 1001 genomes project for arabidopsis thaliana1001年为拟南芥基因组项目.pdf
- the 2011 acr meeting what's new in the windy city2011年acr会议在风城有什么新鲜事.pdf
- the 2003 australian breast health survey survey design and preliminary results2003年澳大利亚乳房健康调查的调查设计和初步结果.pdf
- the 14-3-3s的14-3-3s.pdf
- the aaa+ superfamily of functionally diverse proteins功能多样的蛋白质的aaa +总科.pdf
- the 26th congress of the scandinavian society of anaesthesiology and intensive care medicine, tromso, norway, 13-17 june 2001第26届国会的北欧社会麻醉学和重症监护医学,特罗姆瑟,挪威,2001年6月.pdf
- the chemotactic defect in wiskott-aldrich syndrome macrophages is due to the reduced persistence of directional protrusionswiskott-aldrich综合症巨噬细胞的趋化现象的缺陷是由于定向突起的持久性.pdf
- the chernobyl tissue bank — a repository for biomaterial and data used in integrative and systems biology modeling the human response to radiation切尔诺贝利组织银行u2014u2014存储库中使用的生物材料和数据综合和系统生物学建模人类应对辐射.pdf
- the chromatin remodeler splayed regulates specific stress signaling pathways染色质重塑舒展调节特定压力信号通路.pdf
- the chernobyl childhood leukemia study background & lessons learned切尔诺贝利儿童白血病研究背景和经验教训.pdf
- the choice of the intravenous fluid influences the tolerance of acute normovolemic anemia in anesthetized domestic pigs静脉液体的选择影响急性normovolemic贫血的公差在国内猪麻醉.pdf
- the chromatin remodeling factor smarcb1 forms a complex with human cytomegalovirus proteins ul114 and ul44染色质重塑因子smarcb1形成一个复杂的人类巨细胞病毒蛋白质ul114和ul44.pdf
- the chitinolytic activities of streptomyces sp. th-11链霉菌属的chitinolytic活动th-11 sp.pdf
- the ciliate paramecium shows higher motility in non-uniform chemical landscapes纤毛虫草履虫显示高运动性非均匀化学景观.pdf
- the cinnamon-derived dietary factor cinnamic aldehyde activates the nrf2-dependent antioxidant response in human epithelial colon cells肉桂醛的cinnamon-derived膳食因素激活nrf2-dependent人类结肠上皮细胞抗氧化反应.pdf
- the chromosomal passenger complex activates polo kinase at centromeres在着丝粒染色体乘客复杂激活马球激酶.pdf
文档评论（0）