原创力文档the critical role of n- and c-terminal contact in protein stability and folding of a family 10 xylanase under extreme conditionsn - c端接触的关键作用蛋白质稳定性和折叠的家庭10在极端条件下木聚糖酶.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
the critical role of n- and c-terminal contact in protein stability and folding of a family 10 xylanase under extreme conditionsn - c端接触的关键作用蛋白质稳定性和折叠的家庭10在极端条件下木聚糖酶
The Critical Role of N- and C-Terminal Contact in Protein
Stability and Folding of a Family 10 Xylanase under
Extreme Conditions
1,2 1 2 3
Amit Bhardwaj , Sadhu Leelavathi , Sudeshna Mazumdar-Leighton , Amit Ghosh , Suryanarayanarao
4,5 1
Ramakumar , Vanga S. Reddy *
1 International Centre for Genetic Engineering and Biotechnology, New Delhi, India, 2 Department of Botany, University of Delhi, Delhi, India, 3 National Institute of
Cholera and Enteric Diseases, Kolkata, India, 4 Department of Physics, Indian Institute of Science, Bangalore, India, 5 Bioinformatics Centre, Indian Institute of Science,
Bangalore, India
Abstract
Background: Stabilization strategies adopted by proteins under extreme conditions are very complex and involve various
kinds of interactions. Recent studies have shown that a large proportion of proteins have their N- and C-terminal elements
in close contact and suggested they play a role in protein folding and stability. However, the biological significance of this
contact remains elusive.
Methodology: In the present study, we investigate the role of N- and C-terminal residue interaction using a family 10
xylanase (BSX) with a TIM-barrel structure that shows stability under high temperature, alkali pH, and protease and SDS
treatment. Based on crystal structure, an aromatic cluster was identified that involves Phe4, Trp6 and Tyr343 holding the N-
and C-terminus together; this is a unique and important feature of this protein that might be crucial for folding and stability
under poly-extreme conditions.
Conclusion: A series of mutants was created to disrupt this aromatic cluster formation and study the loss of stability and
function u
您可能关注的文档
- the association between blood pressure and whole blood methylmercury in a cross-sectional study among inuit in greenland血压之间的关系和全血中甲基汞的横断面研究在格陵兰岛的因纽特人.pdf
- the association between armed conflict, violence and mental health a cross sectional study comparing two populations in cundinamarca department, colombia协会之间的武装冲突、暴力和心理健康一个横断面研究比较两个种群cundinamarca部门,哥伦比亚.pdf
- the assessment of population exposure to chlorination by-products a study on the influence of the water distribution system人口评估暴露在氯化消毒副产物对配水系统的影响研究.pdf
- the association between bullying and early stages of suicidal ideation in late adolescents in greece欺凌和自杀意念的早期阶段之间的关系在青少年晚期希腊.pdf
- the association between cardiovascular disease risk and parental educational level in portuguese children心血管疾病风险之间的关系和父母的教育水平在葡萄牙的孩子.pdf
- the association between delusional-like experiences, and tobacco, alcohol or cannabis use a nationwide population-based surveydelusional-like经验之间的关系,和烟草,酒精和大麻使用全国人口调查.pdf
- the association between disease activity and nt-probnp in 238 patients with rheumatoid arthritis a 10-year longitudinal study疾病活动之间的关系,类风湿性关节炎患者中位数水平以上病人在238年10年的纵向研究.pdf
- the association between enterovirus 71 infections and meteorological parameters in taiwan肠病毒71感染和气象参数之间的关系.pdf
- the association between drospirenone and hyperkalemia a comparative-safety study屈和血钾过高相对安全的研究之间的联系.pdf
- the association between genetic variations of chi3l1, levels of the encoded glycoprotein ykl-40 and the lipid profile in a danish populationchi3l1基因变异之间的联系,水平的编码糖蛋白ykl-40丹麦人口和血脂.pdf
- the criteria to confirm the role of epstein-barr virus in nasopharyngeal carcinoma initiation标准确认巴尔病毒在鼻咽癌启蒙的作用.pdf
- the creation and physiological relevance of divergent hydroxylation patterns in the flavonoid pathway不同的羟基化模式的创建和生理相关性类黄酮途径.pdf
- the crucial role of divalent metal ions in the dna-acting efficacy and inhibition of the transcription of dimeric chromomycin a3二价金属离子的至关重要的作用在dna-acting疗效和抑制转录的二聚的色霉素a3.pdf
- the cryptosporidium parvum transcriptome during in vitro development隐孢子虫的以及在体外转录组发展.pdf
- the crystal and molecular structure of (2z)-2-[3-(4-methoxybenzoyl)-4,4-dimethyl-1,2-oxazolidin-2-ylidene]-1-(4-methoxyphenyl)ethanone的晶体和分子结构(2 z)2 -[3 -(4-methoxybenzoyl)4、4-dimethyl-1 2-oxazolidin-2-ylidene]1 -(4-methoxyphenyl)ethanone.pdf
- the cryo-em structure of a complete 30s translation initiation complex from escherichia coli30年代的低温电子显微镜结构完整的翻译起始复合物从大肠杆菌.pdf
- the costs of dominance testosterone, cortisol and intestinal parasites in wild male chimpanzees的成本优势睾酮、皮质醇和肠道寄生虫在野生雄性黑猩猩.pdf
- the cryptochromes中一种的.pdf
- the crystal structure of oprg from pseudomonas aeruginosa, a potential channel for transport of hydrophobic molecules across the outer membraneoprg从铜绿假单胞菌的晶体结构,一个潜在的渠道运输外膜的疏水分子.pdf
- the critically ill patient after hepatobiliary surgery肝胆管的手术后危重病人.pdf
文档评论（0）