the effect of synovial fluid enzymes on the biodegradability of collagen and fibrin clots滑液的影响酶的生物降解性胶原蛋白和纤维蛋白凝块.pdfVIP

Materials 2011, 4, 1469-1482; doi:10.3390/ma4081469 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Article The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots Matthew Palmer, Elizabeth Stanford and Martha M. Murray * Department of Orthopaedic Surgery, Children’s Hospital of Boston, Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA; E-Mails: matthew.palmer@ (M.P.); stanford.elizabeth@ (E.S.) * Author to whom correspondence should be addressed; E-Mail: martha.murray@; Tel.: +1-617-355-7132; Fax: +1-617-730-0459. Received: 18 July 2011; in revised form: 5 August 2011 / Accepted: 12 August 2011 / Published: 22 August 2011 Abstract: Recently there has been a great deal of interest in the use of biomaterials to stimulate wound healing. This is largely due to their ability to centralize high concentrations of compounds known to promote wound healing at a needed location. Joints present a unique challenge to using scaffolds because of the presence of enzymes in synovial fluid which are known to degrade materials that would be stable in other parts of the body. The hypothesis of this study was that atelocollagen scaffolds would have greater resistance to enzymatic degradation than scaffolds made of gelatin, fibrin and whole blood. To test this hypothesis, collagen and fibrin-based scaffolds were placed in matrix metallopeptidase-1 (MMP-1), elastase, and plasmin solutions a