the effects of aging on the molecular and cellular composition of the prostate microenvironment老化的影响前列腺癌的分子和细胞成分微环境.pdfVIP

The Effects of Aging on the Molecular and Cellular Composition of the Prostate Microenvironment 1 2 1 3 3 Daniella Bianchi-Frias , Funda Vakar-Lopez , Ilsa M. Coleman , Stephen R. Plymate , May J. Reed , Peter S. Nelson1,2,3* 1 Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, 2 Department of Pathology, University of Washington, Seattle, Washington, United States of America, 3 Department of Medicine, University of Washington, Seattle, Washington, United States of America Abstract Background: Advancing age is associated with substantial increases in the incidence rates of common diseases affecting the prostate gland including benign prostatic hyperplasia (BPH) and prostate carcinoma. The prostate is comprised of a functional secretory epithelium, a basal epithelium, and a supporting stroma comprised of structural elements, and a spectrum of cell types that includes smooth muscle cells, fibroblasts, and inflammatory cells. As reciprocal interactions between epithelium and stromal constituents are essential for normal organogenesis and serve to maintain normal functions, discordance within the stroma could permit or promote disease processes. In this study we sought to identify aging-associated alterations in the mouse prostate microenvironment that could influence pathology. Methodology/Principal Findings: We quantitated transcript levels in microdissected glandular-adjacent stroma from young (age 4 months) and old (age 20–24 months) C57BL/6 mice, and identified a significant change in the expression of 1259 genes (p,0.05). These included increases in transcripts encoding proteins