use of confocal laser as light source reveals stomata-autonomous function使用共焦激光作为光源显示stomata-autonomous函数.pdfVIP

Use of Confocal Laser as Light Source Reveals Stomata- Autonomous Function ˜ 1 ´ 1,2,3 2,3 1 Roberto C. Canamero , Hernan Boccalandro , Jorge Casal , Laura Serna * ´ ´ 1 Environmental Sciences Faculty, University of Castilla-La Mancha, Toledo, Spain, 2 Instituto de Investigaciones Fisiologicas y Ecologicas Vinculadas ´ a la Agricultura (IFEVA), Faculty of Agronomy, University of Buenos Aires, Buenos Aires, Argentina, 3 Consejo Nacional de Investigaciones Cientıficas y ´ Tecnicas, Buenos Aires, Argentina In most terrestrial plants, stomata open during the day to maximize the update of CO2 for photosynthesis, but they close at night to minimize water loss. Blue light, among several environmental factors, controls this process. Stomata response to diverse stimuli seems to be dictated by the behaviour of neighbour stomata creating leaf areas of coordinated response. Here individual stomata of Arabidopsis leaves were illuminated with a short blue-light pulse by focusing a confocal argon laser. Beautifully, the illuminated stomata open their pores, whereas their dark-adapted neighbours unexpectedly experience no change. This induction of individual stomata opening by low fluence rates of blue light was disrupted in the phototropin1 phototropin2 (phot1 phot2) double mutant, which exhibits insensitivity of stomatal movements in blue-illuminated epidermal strips. The irradiation of all epidermal cells making direct contact with a given stoma in both wild type and phot1 phot2 plants does not trigger its movement. These res