using bacterial extract along with differential gene expression in acropora millepora larvae to decouple the processes of attachment and metamorphosis使用细菌提取基因差异表达以及在鹿角幼虫分离附件和蜕变的过程.pdfVIP

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using bacterial extract along with differential gene expression in acropora millepora larvae to decouple the processes of attachment and metamorphosis使用细菌提取基因差异表达以及在鹿角幼虫分离附件和蜕变的过程.pdf

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using bacterial extract along with differential gene expression in acropora millepora larvae to decouple the processes of attachment and metamorphosis使用细菌提取基因差异表达以及在鹿角幼虫分离附件和蜕变的过程

Using Bacterial Extract along with Differential Gene Expression in Acropora millepora Larvae to Decouple the Processes of Attachment and Metamorphosis 1 1 1 1 1 2 Nachshon Siboni *, David Abrego , Francois Seneca , Cherie A. Motti , Nikos Andreakis , Jan Tebben , Linda L. Blackall1, Tilmann Harder1,2 1 Australian Institute of Marine Science, Townsville, Australia, 2 School of Biological, Earth and Environmental Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney, Australia Abstract Biofilms of the bacterium Pseudoalteromonas induce metamorphosis of acroporid coral larvae. The bacterial metabolite tetrabromopyrrole (TBP), isolated from an extract of Pseudoalteromonas sp. associated with the crustose coralline alga (CCA) Neogoniolithon fosliei, induced coral larval metamorphosis (100%) with little or no attachment (0–2%). To better understand the molecular events and mechanisms underpinning the induction of Acropora millepora larval metamorphosis, including cell proliferation, apoptosis, differentiation, migration, adhesion and biomineralisation, two novel coral gene expression assays were implemented. These involved the use of reverse-transcriptase quantitative PCR (RT-qPCR) and employed 47 genes of interest (GOI), selected based on putative roles in the processes of settlement and metamorphosis. Substantial differences in transcriptomic responses of GOI were detected following incubation of A. millepora larvae with a threshold concentration and 10-fold elevated concentration of TBP-containing extracts of Pseudoalteromonas sp. The notable and relatively abrupt changes of the larval body structure during metamorphosis correlated, at the molecular level,