viral discovery and sequence recovery using dna microarrays病毒的发现和序列恢复使用dna微阵列.pdfVIP

PLoS BIOLOGY Viral Discovery and Sequence Recovery Using DNA Microarrays 1 1 2 1 3 3 David Wang , Anatoly Urisman , Yu-Tsueng Liu , Michael Springer , Thomas G. Ksiazek , Dean D. Erdman , 4 4 4 4 4 4 Elaine R. Mardis , Matthew Hickenbotham , Vincent Magrini , James Eldred , J. Phillipe Latreille , Richard K. Wilson , 2 1* Don Ganem , Joseph L. DeRisi 1 Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America, 2 Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America, 3 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 4 Department of Genetics, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, United States of America Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification and characterization. Central to this approach was a DNA microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the most highly conserved 70mer sequences from every fully sequenced reference viral genome in GenBank. During an outbreak