use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant escherichia coli using pcr and future prospects with other bacterial genera a pilot study英文论文.pdfVIP

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use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant escherichia coli using pcr and future prospects with other bacterial genera a pilot study英文论文.pdf

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use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant escherichia coli using pcr and future prospects with other bacterial genera a pilot study英文论文

Advances in Biological Chemistry, 2012, 2, 366-371 ABC /10.4236/abc.2012.24045 Published Online November 2012 (http://www.SciRP.org/journal/abc/) Use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant Escherichia coli using PCR and future prospects with other * bacterial genera: A pilot study 1,2# 2 3 Subramanian Krishnan , Dheepikaa Balasubramanian , B. Appala Raju , Baddireddi Subadhra Lakshmi2 1Division of Infectious Diseases, Department of Pediatrics, The Saban Research Institute, Children’s Hospital Los Angeles, Univer- sity of Southern California School of Medicine, Los Angeles, USA 2Tissue Culture and Drug Discovery Laboratory, Centre for Biotechnology, Anna University, Chennai, India 3Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, India Email: #skrishnan@ Received 12 September 2012; revised 14 October 2012; accepted 21 October 2012 ABSTRACT stance [6]. Similarly, aspartate at residue 87 (asp87) is another important target for quinolone drugs. Mutation We developed a novel PCR method aimed at identi- of asp87 in gyrase A to asparagine (Asp) or valine (Val) fying and amplifying native codon sequences of muta- is common [7]. But in E. coli, the serine codon for pri- tion-prone amino acids in DNA gyrase implicated in