原创力文档use of cell-selex to generate dna aptamers as molecular probes of hpv-associated cervical cancer cells使用cell-selex生成dna寡核苷酸适配子hpv-associated宫颈癌细胞的分子探针.pdfVIP
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use of cell-selex to generate dna aptamers as molecular probes of hpv-associated cervical cancer cells使用cell-selex生成dna寡核苷酸适配子hpv-associated宫颈癌细胞的分子探针
Use of Cell-SELEX to Generate DNA Aptamers as
Molecular Probes of HPV-Associated Cervical Cancer
Cells
1 1,2,3
Jessica C. Graham , Helmut Zarbl *
1 Department of Environmental and Occupational Medicine, Robert Wood Johnson, Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New
Jersey, United States of America, 2 Program in Carcinogenesis and Chemoprevention, Division of Public Health Sciences, Cancer Institute of New Jersey, New Brunswick,
New Jersey, United States of America, 3 NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, University of
Medicine and Dentistry of New Jersey, Piscataway, New Jersey, United States of America
Abstract
Background: Disease-specific biomarkers are an important tool for the timely and effective management of pathological
conditions, including determination of susceptibility, diagnosis, and monitoring efficacy of preventive or therapeutic
strategies. Aptamers, comprising single-stranded or double-stranded DNA or RNA, can serve as biomarkers of disease or
biological states. Aptamers can bind to specific epitopes on macromolecules by virtue of their three dimensional structures
and, much like antibodies, aptamers can be used to target specific epitopes on the basis of their molecular shape. The
Systematic Evolution of Ligands by EXponential enrichment (SELEX) is the approach used to select high affinity aptamers for
specific macromolecular targets from among the .1013 oligomers comprising typical random oligomer libraries. In the
present study, we used live cell-based SELEX to identify DNA aptamers which recognize cell surface differences between
HPV-transformed cervical carcinoma cancer cells and isogenic, nontumorigenic, revertant cell lin
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