vitellogenin functions as a multivalent pattern recognition receptor with an opsonic activity卵黄蛋白原功能作为一种多价模式识别受体调理素的活动.pdfVIP

Vitellogenin Functions as a Multivalent Pattern Recognition Receptor with an Opsonic Activity 1 1 2 Zhaojie Li , Shicui Zhang *, Qinghui Liu 1 Department of Marine Biology, Ocean University of China, Qingdao, People’s Republic of China, 2 Yellow Sea Fishery Research Institute, Chinese Academy of Fisheries, Qingdao, People’s Republic of China Abstract Background: Vitellogenin (Vg), a major reproductive protein, has been associated with infection-resistant response in fish. However, the underlying mechanisms by which Vg is involved in anti-infectious response are not understood. Methodology/Results: By both protein-microbe interaction analysis and enzyme-linked immunosorbent assay as well as phagocytosis test, we demonstrate for the first time that fish Vg acts as a pattern recognition molecule with multiple specificities that can recognize bacteria as well as fungus rather than self components from fish, and functions as an opsonin that can enhance macrophage phagocytosis. Conclusions: This study shows that fish Vg plays an integrative function in regulating immunity via its pleiotropic effects on both recognizing pathogen-associated molecular patterns and promoting macrophage phagocytosis. It also supports the notion that factors normally involved in control of female reproduction are associated with immunity in organisms that rely on Vg for oocyte development. Citation: Li Z, Zhang S, Liu Q (2008) Vitellogenin Functions as a Multivalent Pattern Recognition Receptor with an Opsonic Activity. PLoS ONE 3(4): e1940. doi:10.1371/journal.pone.0001940 Editor: Robin Charles May, University of Birmingham, United Kingdom Received November 20, 2007; Accepted February 29, 2008; Published April 9, 2008 Copyright: 2008 Li et al. This is an open-access articl