vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application脆弱性肠道猪上皮细胞分化的霉菌毒素deoxynivalenol取决于应用程序的路径.pdfVIP

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vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application脆弱性肠道猪上皮细胞分化的霉菌毒素deoxynivalenol取决于应用程序的路径.pdf

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vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application脆弱性肠道猪上皮细胞分化的霉菌毒素deoxynivalenol取决于应用程序的路径

Vulnerability of Polarised Intestinal Porcine Epithelial Cells to Mycotoxin Deoxynivalenol Depends on the Route of Application 1 1 ¨ 2 1 1 1 Anne-Kathrin Diesing , Constanze Nossol , Sven Danicke , Nicole Walk , Andreas Post , Stefan Kahlert , ¨ 1 1 Hermann-Josef Rothkotter , Jeannette Kluess * 1 Medical Faculty, Institute of Anatomy, Otto-von-Guericke University, Magdeburg, Germany, 2 Institute of Animal Nutrition, Federal Research Institute for Animal Health, Braunschweig, Germany Abstract Background and Aims: Deoxynivalenol (DON) is a Fusarium derived mycotoxin, often occurring on cereals used for human and animal nutrition. The intestine, as prominent barrier for nutritional toxins, has to handle the mycotoxin from the mucosa protected luminal side (apical exposure), as well as already absorbed toxin, reaching the cells from basolateral side via the blood stream. In the present study, the impact of the direction of DON exposure on epithelial cell behaviour and intestinal barrier integrity was elucidated. Methods: A non-transformed intestinal porcine epithelial cell line (IPEC-J2), cultured in membrane inserts, serving as a polarised in vitro model to determine the effects of deoxynivalenol (DON) on cellular viability and tight junction integrity. Results: Application of DON in concentrations up to 4000 ng/mL for 24, 48 and 72 hours on the basolateral side of membrane cultured polarised IPEC-J2 cells resulted in a breakdown of the integrity of cell connections measured by transepithelial electrical resistance (TEER), as well as a reduced expression of the tight junction proteins ZO-1 and claudin 3. Epithelial cell