dnaemia detection by multiplex pcr and biomarkers for infection in systemic inflammatory response syndrome patientsdnaemia多重pcr和生物标志物的检测感染全身炎症反应综合征患者.pdfVIP

DNAemia Detection by Multiplex PCR and Biomarkers for Infection in Systemic Inflammatory Response Syndrome Patients 1 1 1 2 3 Catherine Fitting , Marianna Parlato , Minou Adib-Conquy , Nathalie Memain , Franc¸ois Philippart , ˆ 3, Mehran Monchi4, Jean-Marc Cavaillon 1*, Christophe Adrie5 Benoıt Misset 1 Unit Cytokines Inflammation, Department of Infection and Epidemiology, Institut Pasteur, Paris, France, 2 Service de reanimation, Delafontaine Hospital, Saint Denis, ´ ´ ´ France, 3 Service de reanimation medico-chirurgicale, Groupe hospitalier Paris Saint-Joseph, Universite Paris Descartes, Paris, France, 4 Service de Soins Intensifs, Jacques Cartier Hospital, Massy-Palaiseau, France, 5 Service de Physiologie et d’exploration fonctionnelles, Cochin Hospital, University of Paris Descartes, Sorbonne cite, Paris, France Abstract Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOOH) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using