赛莱西布对肝癌HepG2细胞增殖及凋亡影响.docVIP

赛莱西布对肝癌HepG2细胞增殖及凋亡影响 　【关键词】 ,HepG2细胞;环氧合酶-2；赛莱西布；增殖;凋亡 　　摘要： 目的 探讨选择性环氧合酶-2(cox-2)抑制剂赛莱西布(celecoxib)对人肝癌细胞株HepG2细胞增殖、凋亡的影响及其可能的作用机制。方法 用不同浓度的赛来西布处理HepG2细胞，分别应用四甲基偶氮噻唑蓝试验、DNA梯度电泳法、放射免疫法检测赛亚西布对HepG2细胞增殖和凋亡及其cox-2活性的影响；应用免疫印迹法和比色法检测赛莱西布对HepG2细胞胱氨酸蛋白酶3(clasepase-3)蛋白质的表达及活性的影响。结果 125，25，50μmol/L的赛莱西布作用于HepG2细胞后，HepG2细胞增殖受抑制，与对照组比较，差异有统计学意义(Plt;001)；赛莱西布干预组HepG2细胞DNA明显降解，可见细胞凋亡特征性梯状DNA条带；干预组HepG2细胞caspase3蛋白表达及活性均明显升高，与对照组比较，差异均有统计学意义(Plt;001)；赛莱西布明显抑制HepG2细胞cox-2活性，与对照组比较，差异有统计学意义(Plt;001)。结论 赛莱西布可通过cox-2通路和caspase3通路抑制HepG2细胞增殖并诱导其凋亡。 　　关键词： HepG2细胞;环氧合酶-2；赛莱西布；增殖;凋亡 　　Effects of cyclooxygenase-2 on proliferation and apoptosis in HepG2 cells 　　Abstract： Objective To examine the effects of a selective inhibitor of cox-2,celecoxib on growth and apoptosis in HepG2 cells.Methods HepG2 cells were treated with various concentrations of celecoxib.Cell growth was measured by MTT,apoptosis was detected by DNA fragmentation assay,and cox-2 activity was measured by RIA;Expression of caspase-3 protein in HepG2 cells was determined by western blot,and caspase-3 activity was measured using colorimetric assay.Results After HepG2 cells were treated with different concentrations of celecoxib (12.5,25,50μmol/L),celecoxib could significantly suppress the growth of HepG2 cells respectively compared with the control group (Plt;0.01),and celecoxib-treated HepG2 cells produced a distinct oligosomal ladder,the characteristics of cells undergoing apoptosis;Meanwhile,The caspas-3 expression and activity of celecoxib treated HepG2 cells were increased respectively as compared with the control groups(Plt;0.01).Furthermore,the level of cox-2 activity was reduced in celecoxib-treated HepG2 cells respectively compared with the control group (Plt;0.01).Conclusion Celecoxib could inhibit the proliferation and increase apoptosis in HepG2 cells by cox-2 and caspase3 pathways. 　　Key words： HepG2 cell;cyclooxygenase-2;celeeoxib;proliferation;apoptosis 　　近年来的研究表明，环氧合酶-2(cox-2)在结直肠癌、食管癌、肺癌等多种人类肿瘤组织中高表达〔