通冠胶囊对大鼠颈动脉球囊损伤术后血管内膜VEGF表达影响.docVIP

通冠胶囊对大鼠颈动脉球囊损伤术后血管内膜VEGF表达影响 　作者：訾勇 张敏州 王磊 郭力恒 张军 温春瑜 杨澄 【摘要】 目的探讨通冠胶囊对血管损伤后内膜增生及VEGF表达的影响。方法制备大鼠左颈总动脉球囊损伤模型，将54只SD大鼠随机分为通冠胶囊组、盐水对照组和辛伐他汀对照组，于术后第4周、第8周和第12周处死动物。分别进行取材检测指标：HE染色及弹力纤维染色后光镜下观察管壁增生情况，计算机图像分析内、中膜面积及其比值观察通冠胶囊对新生内膜形成的影响。血管内皮生长因子(VEGF)免疫组织化学法检测明确损伤后血管内皮修复情况。结果通冠胶囊组在4周、8周和12周时免疫组化VEGF表达较盐水对照组明显升高(均Plt;0.01)，辛伐他汀组的变化趋势与通冠胶囊组相似。通冠胶囊组4周、8周与同时段辛伐他汀组比较无统计学差异(均Pgt;0.05)，12周较辛伐他汀组明显升高(Plt;0.05)。球囊损伤后4周各组内膜增生明显，8周时形成的增生内膜减少，12周时形成的增生内膜明显减少。通冠胶囊组在损伤后4周、8周、12周形成的增生内膜较盐水对照组显著减少(均Plt;0.01)，内膜面积与中膜面积的比值较盐水对照组明显降低(均Plt;0.01)。结论通冠胶囊可以促进大鼠颈总动脉球囊损伤处的VEGF的表达，并减少新生内膜的形成。 【关键词】 通冠胶囊; 大鼠颈动脉; 球囊损伤; 血管内膜; 血管内皮生长因子 　　Abstract：ObjectiveTo observe the effects of Tongguan capsule on the expression of vascular endothelial growth factor and intimal hyperplasy in Tunica intima in rats after balloon injury.Methods60 male Sprague-Dawley rats whose left common carotid arteries were injured were randomly divided into 3 groups (n=18) :Group l(10mg·kg-1·d-1 of PS was given after injury)，group 2(2mg·kg-1·d-1 of Simvastatin was given after injury)and group 3(600mg·kg-1·d-1 of Tongguan capsule was given after injury). 18 rats from each group were euthanized by exsanguination in week 4,week 8 and week 12 after injury .The injured left common carotid arteries were carefully removed for HE and elastic fibers staining.Intima area,media area and IA/MA were performed in IPP6.0.which is an automatic computerized imaging analysis system.To observe the reparation after injury of blood vessel endothelium through detection of VEGF with immunohistochemistry.ResultsIn comparison with Group l, the expression of VEGF in tunica intima in rats after balloon injury in Group 3 increased obviously(Plt;0.01).The changes of the expression of VEGF of Group 2 were similar to Group 3. The expression of VEGF of Group 3 increased obviously in week 12(Plt;0.05).4 weeks after the operation，morphological analysis demonstrated that there were marked differences in the area of neointima proliferation among all the groups.Concl