金黄色葡萄球菌耐消毒剂基因qacA探究.docVIP

金黄色葡萄球菌耐消毒剂基因qacA探究 　 【摘要】; 目的 检测我院近年来临床分离的金葡菌耐消毒剂的qacA基因水平，为动态监测细菌耐药性的变化趋势、有效控制细菌耐药性的传播提供对策。方法 收集我科临床分离的金葡菌共252株，并经本实验室重新鉴定确认。参照美国CLSI/NCCLS标准，用6μg/ml苯唑西林和4% NaCl平板，筛选出耐苯唑西林金葡菌和对苯唑西林敏感的金葡菌。应用PCR基因扩增试验检测金葡菌中耐消毒剂的qacA基因水平。随机抽取一株MRSA阳性株进行基因测序，作qacA基因验证。作qacA基因阳性株的MIC检测，了解阳性株是否有耐药性的表达。结果 共检测金葡菌252株，其中MRSA 84株(带有qacA基因4株)，MSSA 168株(带有qacA基因1株)。MRSA阳性菌株对消毒剂的MIC值明显高于MSSA菌株。结论 携带含有耐消毒剂基因qacA的金葡菌未在我院流行。 毕业论文 【关键词】; 金黄色葡萄球菌 消毒剂 qacA基因 毕业论文 ; Study on the disinfectant?resistant gene qacA in Staphylococcus aureus 毕业论文 ; ABSTRACT; Objective; To investigate the disinfectant?resistant gene qacA in Staphylococcus aureus isolates from the First Affiliated Hospital of Chongqing Medical University and control drug?resistance transmission of bacteria.; Method; Two hundred and fifty?two Staphylococcus aureu isolates were collected and reconfirmed by our laboratory. The agar plate containing oxacillin 6μg/ml and 4% NaCl was used to select methicillin?susceptible Staphylococcus aureus and methicillin?resistant Staphylococcous aureus, according to the standard recommended by CLSI/NCCLS (2005) of USA. The qacA gene in the isolates were amplified by PCR. The PCR products were sequenced for verification with the object sequence of GenBank. The drug?susceptible test was performed in qacA carrying stains.; Results; Among 252 Staphylococcous aureus isolates, only 4 strains were found qacA gene in 84 MRSA and 1 strain in 168. The MICs of antiseptics were higher against MRSA than MSSA.; Conclusion; Disinfectant?resistant gene qacA ?carrying Staphylococcus aureus hasn′t previled in the hospital. 毕业论文 ; KEY WORDS; Staphylococcus aureus Disinfectants qacA gene 毕业论文 ; 金黄色葡萄球菌(Staphylococcus aureus)是临床上最常见的致病菌之一，其产生的毒素和酶最多，毒力在葡萄球菌中最强，尤其耐甲氧西林金葡菌(methicillin?resistant Staphylococcus aureus，MRSA)是医院内感染的主要病原菌，MRSA具有多重耐药特征，是临床抗感染治疗的难题之一。20世纪80年代国外学者已在金葡菌中检测到耐消毒剂基因qacA，2005年我国也从多重耐药MRSA中发现耐消毒剂基因qacA的报道［1］。 毕业论文 ; 金葡菌对消毒剂的耐药基因命名来源于其对季胺类化合物(quaternary ammonium compounds，qac)的耐药，主要由qac