雷公藤多甙对急性移植物抗宿主病小鼠作用.docVIP

雷公藤多甙对急性移植物抗宿主病小鼠作用 　作者：于艳秋，张义侠，金玉楠，卢晓梅，王巍，张海鹏 毕业论文 【摘要】; 本研究应用雷公藤多甙防治小鼠急性移植物抗宿主病。对受致死量照射的C57BL/6受鼠，注入供鼠BABC/c骨髓与脾淋巴细胞的混合液，并给予雷公藤多甙、环孢素A、甲氨蝶呤处理。用免疫组织化学法、流式细胞术和ELISA法检测各组小鼠T淋巴细胞、黏附分子、细胞因子。结果表明： 所有异基因对照组小鼠，在30天内死于aGVHD,而雷公藤多甙组（19/21）、环孢素A+甲氨蝶呤组（13/21）和雷公藤多甙+环孢素A组（17/21）的大部分小鼠存活时间超过了30天，并没有明显的aGVHD症状表现。雷公藤多甙、环孢素A、甲氨蝶呤可明显降低皮肤、肺组织CD3+、CD4+、CD8+、CD11a+、CD18+细胞（Plt;0.05）和脾组织CD3+, CD4+, CD8+, CD4+CD11a+, CD4+CD18+, CD8+CD11a+ , CD8+CD18+细胞（Plt;0.05）。而小肠组织CD3+、CD4+、CD8+细胞变化不明显（Pgt;0?05）。同时，雷公藤多甙可降低受鼠血清中IL-2、TNFalpha;浓度和脾组织IL-2、TNFalpha;的mRNA的表达（Plt;0.05）；上调血清中IL-10的水平（Plt;0.05），但对IL-4的作用不显著（Pgt;0.05）。结论：雷公藤多甙具有明显抗aGVHD作用并同时保留抗白血病效应，其作用机制与调节促炎/抑炎细胞因子的分泌和黏附分子的表达，以及抑制T淋巴细胞作用有关。 毕业论文 【关键词】; 雷公藤多甙；移植物抗宿主病； T淋巴细胞； 黏附分子；细胞因子 毕业论文 　　　Glucosidorum Tripterygii Tororum (GTT) is a kind of Chinese traditional medicine (CTM), for the first time used in China as an immunoregulatory agent which is often used in the treatment of autoimmunological diseases such as rheumatoid arthritis (RA) and nephrotic syndrome. Our previous experiment showed that GTT can decrease the population of lymphocytes, inhibit the secretion of IL-2 and expression of IL-2R, down-regulate the sensitivity of T cells to IL-2, and inhibit the expression of adhesion molecules (CD11a, CD18) on the surface of T cells. And it can prolong the survival; time of allogeneic transplanted skin. Its effect on anti-rejection is weaker than CsA and stronger than methylprednisolone. And, the combination of GTT with them shows a much more stronger effect, indicating that GTT can moderately or obviously inhibit the function of T cells and the secretion of cytokines［1］. Furthermore, GTT may have anti-inflammatory and anti-tumor effects. Graft-Versus-Host disease (GVHD), especially acute Graft-Versus-Host disease (aGVHD) is a major lethal complication after bone marrow transplantation (BMT). Therefore, this study was aimed to adopt GTT or combine GTT with CsA; in order; to explore the role of GTT on aGVHD and its mechanism. 毕业论文