靶向血管紧张素Ⅱ受体基因短发夹RNA功能比较.docVIP

靶向血管紧张素Ⅱ受体基因短发夹RNA功能比较 　 作者：邱龄，肖传实，曾秋棠，李茂莲 【关键词】 RNA干扰；高血压；血管紧张素Ⅱ受体；载体 　　 　　Gene silencing targeted to the rat AT1 receptors by efficient shRNAs and its screening 【Abstract】 AIM: To investigate the efficacy of short hairpin RNAs (shRNAs) to modulate the expression of rat angiotensin Ⅱ type1 (AT1) receptor gene in mammalian cells and to explore the correlations between the features of efficient shRNAs and shRNAs functionality. METHODS: We generated 4 shRNAs expression vectors targeting against rat angiotensinⅡ receptors. The shRNAs were targeted to 4 different regions of the same gene. The ra