CpG ODN联合HBsAg免疫BALB-c小鼠及HBV转基因C57BL-6J小鼠.doc

CpG ODN联合HBsAg免疫BALB/c小鼠及HBV转基因C57BL/6J小鼠 　【关键词】 乙型 CpG oligodeoxyribonucleotide with hepatitis B surface antigen (HBsAg) for vaccination in BALB/c and HBVtransgenic mice 　　【Abstract】AIM： To study the effects of CpG oligodeoxyribonucleotide (ODN) as adjuvant on the immune responses in BALB/c and HBV-transgenic mice with hepatitis B surface antigen (HBsAg). METHODS: BALB/c and HBV transgenic mice were immunized by multiplesite intramuscular injection with HBsAg and phosphorothioate oligodeoxynucleotides containing two CpG motifs. The HBsAg and antiHBs in serum from immunized mice were detected by ELISA and the number of IFNsecreting T cells was examined by ELISPOT. RESULTS: In BALB/c mice, the mice immunized with CpG ODN plus HBsAg showed higher specific humoral immune responses to HBsAg than those immunized with HBsAg alone. Compared with the immunization with HBsAg or CpG ODN alone, the immunization with HBsAg plus CpG ODN activated respectively 3 or 9 times more HBs-specific IFNsecreting T cells. In HBV transgenic mice, antiHBs in serum were detected in the mice immunized with HBsAg and CpG ODN while no antiHBs in serum in the mice immunized with HBsAg or CpG ODN alone. There was no significant difference in the level of HBsAg in serum between the three groups after vaccination (Pgt;0.05) but the level declined in all three groups compared with those prevaccination (Plt;0.05). Compared with the immunization with HBsAg or CpG ODN alone, the immunization with HBsAg plus CpG ODN activated, respectively, 3 or 11 times more HBsspecific IFNsecreting T cells. CONCLUSION: CpG ODN can act as adjuvant to enhance the humoral and cell immune responses in BALB/c mice immunized with HBsAg. CpG ODN oligodeoxyribonucleotide combined with HBsAg can break the tolerance to this antigen in HBV transgenic mice and may become a potential prophylactic and therapeutic approach. 　　【Keywords】 CpG ODN; hepatitis B surface antigens (HBsAg); mice, inbred BALB/C; mice， transgenic; immune res