L-精氨酸对糖尿病大鼠坐骨神经损伤保护作用.doc

L-精氨酸对糖尿病大鼠坐骨神经损伤保护作用 　 作者：陈秀芳，董敏，金晓冬，雷康福 金丽琴 【摘要】 目的:探讨L-精氨酸（L-Arg）对糖尿病(DM)大鼠坐骨神经损伤的保护作用及其机制。方法:用链脲佐菌素制备糖尿病模型。SD大鼠随机分为正常对照组（NC组）、DM组、L-Arg治疗组（L-Arg组）。L-Arg治疗12周后，测定三组大鼠血清、坐骨神经组织一氧化氮(NO)含量和一氧化氮合酶(NOS)活性及血浆内皮素-1(ET-1)、降钙素基因相关肽(CGRP)含量，以及坐骨神经组织Ca2+-ATPase、Na+-K+-ATPase活性及神经元型NOS（nNOS）、醛糖还原酶（AR）基因的表达。结果:DM组大鼠血清、坐骨神经NO含量和NOS活性、血浆CGRP含量及坐骨神经Ca2+-ATPase、Na+-K+-ATPase活性和nNOS mRNA 的表达均明显低于NC组（Plt;0.01或Plt;0.05），而血浆ET-1含量、坐骨神经AR mRNA的表达均明显高于NC组（均Plt;0.01）；经L-Arg治疗后，上述改变逆转，与DM组比较差异有显著性（Plt;0.01或Plt;0.05）。结论:L-Arg对DM大鼠坐骨神经损伤具有一定的保护作用，其机制可能与改善神经血供及抑制多元醇代谢途径有关。 【关键词】 L-精氨酸；糖尿病；坐骨神经；一氧化氮；内皮素-1；降钙素基因相关肽；醛糖还原酶；大鼠 Abstract: Objective: To investigate the effects and mechanisms of L-arginine (L-Arg) on preventing and treating sciatic nerve injury of experimental diabetic rats. Methods: The diabetes mellitus(DM) rats models were made by intraperitoneal injection of streptozotocin (60 mg/kg body weight), and three groups, including normal control group （NC group）, DM group, L-Arg treatment group (L-Arg group) were allocated at random. After the supplementation of L-Arg (300 mg·kg-1·d-1) for 12 weeks, the rats were sacrificed. The nitric oxide (NO) content and nitric oxide synthase (NOS) activity in serum and sciatic nerve, plasma endothelin-1 (ET-1) and calcitonin gene-related peptide (CGRP) content were determined. The activities of Ca2+-ATPase, Na+-K+-ATPase and the mRNA expression level of neuronal NOS (nNOS) and aldose reductase (AR) of sciatic nerve also were measured. Results: The NO content and NOS activity in serum and sciatic nerve, plasma CGRP content, and Ca2+-ATPase, Na+-K+-ATPase activities and nNOS mRNA expression level in sciatic nerve of DM group were significantly lower than those of NC group (Plt;0.01 or Plt;0.05 respectively), but plasma ET-1 content and AR mRNA expression level in sciatic nerve were significantly higher than those of NC group (Plt;0.01 respectively). But in L-Arg group, the above changes were reversed, there were significant differences compared