三拗芎葶合剂对低氧性肺动脉高压大鼠血清超氧化物歧化酶-丙二醛.docVIP

三拗芎葶合剂对低氧性肺动脉高压大鼠血清超氧化物歧化酶/丙二醛 　 作者：杨仁旭，卢小龙，杜建霖，常生杰，任颖，何祝萍 【摘要】 目的 观察三拗芎葶合剂对HPH大鼠功能形态学及血中SOD和 MDA的影响，并探讨其机制。方法 将50只雄性SD大鼠随机分为5组：正常对照组、模型组、卡托普利组、三拗芎葶合剂中剂量组及三拗芎葶合剂大剂量组，每组10只。利用常压缺氧方法复制大鼠低氧性肺动脉高压模型，2周后灌胃相应药物，连续用药24天后，采用改良右心导管法测定肺血流动力学参数，比色法测定血清SOD水平与MDA含量；处死大鼠后，称量wW、RV和LV+S，计算右心肥厚指数(RV／LV+S)及肺渗出指数(wW／BW)；利用图像分析方法，测定肺小动脉病理及其形态计量学。结果 (1)三拗芎葶合剂及卡托普利均能明显降低模型大鼠mPAP、wW／BW、RV／LV+S(Plt;0.05和Plt;0.01)，改善模型大鼠血流动力学指标，部分逆转模型大鼠的肺血管重构；同时提高血清SOD活性水平，降低血清MDA含量(Plt;0.05和Plt;0.01)，三拗芎葶合剂各剂量组与卡托普利组疗效相当；但治疗组上述各指标仍未完全恢复到正常对照组水平。结论 三拗芎葶合剂能提高血清SOD活性水平，降低血清MDA含量，能有效降低HPH模型大鼠的肺动脉高压，部分逆转肺血管结构重建，改善右心功能，其作用机制可能与清除自由基、抗脂质过氧化，保护EC，维持VSMC的正常舒张功能有关，提示该药将是防治HPH的有效中药复方制剂。 【关键词】 三拗芎葶合剂；低氧性肺动脉高压；超氧化物歧化酶；丙二醛；肺血流动力学 　　【Abstract】 Objective Observation the influence of Sanao Xiongting mixture on hypoxic pulmonary hypertension rats functional morphology and SOD/MDA in serum ,and discussing the mechanism. Methods 50 male Sprague-Dawley rats were randomly divided into five groups : normal control group, model group, captopril group, Sanao Xiongting mixture of mid- dose group and Sanao Xiongting mixture of high-dose group, 10 in each group. Using hypoxia induce pulmonary hypertension, two weeks later gavage corresponding drugs, after 24 days of continuous medication,using modified right heart catheterization to determine the pulmonary hemodynamic parameters, colorimetry was used to determine the serum levels of SOD and MDA; Rats were killed and weighed wW, RV and LV + S, calculated the index of right ventricular hypertrophy (RV / LV + S) and pulmonary exudative index (wW / BW); Using image analysis method to determine the pulmonary arterial pathology and morphometry. Results (1) Sanao Xiongting mixture and captopril both could significantly reduce the rats?mPAP, wW / BW, RV / LV + S (Plt; 0.05 and Plt;0.01), improve rats? index of hemodynamics, partly reverse the pulmonary vascular remodeling; meanwhile, raising the level of activity of serum SOD, reducing the level of MDA (Plt; 0.05 and Plt;0.01), Sanao Xion