保留残迹对前交叉韧带重建移植物愈合影响组织学探究.docVIP

保留残迹对前交叉韧带重建移植物愈合影响组织学探究 　【摘要】 ：［目的］观察保留残迹对前交叉韧带(anterior cruciate ligament，ACL)重建移植物愈合的影响，并探讨其原因。［方法］新西兰兔30只(封闭群，体重2.9～3.5 kg，平均3.2 kg)随机分为3组，每组10只。取其跟腱为移植物，切断双侧前交叉韧带，随机一侧保留ACL残迹，对侧切除残迹，行前交叉韧带重建。分别于术后4、8、12周处死动物，取关节内重建ACL移植物标本10％多聚甲醛固定、10％EDTA脱钙、包埋、切片，HE染色及Masson、PAS特殊组织化学染色，检测成纤维细胞密度及观察组织学形态，数据采用SPSS 12.0软件进行统计分析。［结果］各时间段保留残迹组移植物HE染色组织切片成纤维细胞密度均高于切除残迹组，两组比较有显著统计学差异(Plt;0.05)，两处理组8周时细胞密度高于4、12周，不同时间段比较有统计学差异(Plt;0.05)。各时间段保留残迹组移植物的组织学表现均优于切除残迹组。［结论］保留残迹ACL重建有利于新生细胞长入替代移植物，促进移植物组织结构成熟。 【关键词】 膝关节　前交叉韧带　残迹　重建　愈合 　 Abstract：［Objective］To observe the effects of preserving the remnant on healing of ACL autograft in anterior cruciate ligament (ACL) reconstruction,and discuss the reasons.［Method］Forty closed colony New Zealand rabbits with the average weight of 3.2 kg were divided into 3 groups at random,each group had 10 rabbits.The anterior cruciate ligament of the rabbit was cut off in both knees,the remnant of original ACL was preserved on one side and removed on the opposite side at random,then single bundle anterior cruciate ligament reconstruction was conducted with autogeneous achilles tendon graft.Animals were executed and the intraarticlar graft specimens were taken at 4,8,12,weeks postoperatively respectively.The specimens were fixed with 10 % paraformaldehyde,decalcified with 10% EDTA.The specimens were taken and embeded,sliced,and then strained with HE and Masson,PAS special histochemistry to observe fibroblast density and histopathological situation.Statistical analysis were carried out using SPSS 12.0 software.［Result］In each time section,the fibroblast density of ACL grafts tissue section with HE dyeing in preservingremnant group was higher than that in removingremnant group,there was significant difference between two groups (Plt;0.05).The density of 8 week time section was higher than those of 4 week time section and 12 week time section.There was significant difference among different time sections in each treatment group (Plt;0.05),the histological a