大黄素对大鼠移植肝细胞凋亡作用.docVIP

大黄素对大鼠移植肝细胞凋亡作用 　【摘要】 目的 探讨大黄素对大鼠肝脏移植术后肝细胞凋亡的作用。方法 建立大鼠肝移植模型，分为三组：对照组，供受体均为LEW大鼠；移植组；供体为LEW大鼠，受体为BN大鼠；大黄素组，在移植组基础上，移植术后每日以大黄素50 mg·kg-1腹腔注射。分别于术后第1，3，5，7天各取6只移植大鼠的肝脏，TUNEL法染色，检测肝脏细胞凋亡。以肝脏细胞凋亡阳性细胞数占总肝脏细胞数的百分比作为肝细胞凋亡指数(AI)。结果 移植组肝细胞于术后第1天即已出现凋亡，第3天明显增加，第7天达到高峰。大黄素组各时间点肝细胞凋亡指数明显小于对应的移植组(Plt;0.01)。结论 大黄素对肝移植急性排斥反应中肝细胞凋亡有显著抑制作用。 【关键词】 大黄素 肝移植 移植物排斥 细胞凋亡 大鼠 Abstract Objective To evaluate the effect of Emodin on hepatocellular apoptosis following orthotopic liver transplantation in rats. Methods The model of orthotopic liver transplantation in rats was set up. The rats were randomly divided into three groups. In control group， the donors and receptor were LEW rats； in transplant group， the donors were LEW rats and the receptor were all BN rats； in Emodin group， rats were treated with same agent as those in transplant group done， except that they were fed with additional Emodin after liver transplantation. On the 1st， 3rd， 5th and 7th day after transplantation， apoptosis index was evaluated by the percentage of liver cells with TUNEL positive staining. Results The apoptotic liver cells appeared on the 1st day and significantly increased at the 3rd day， while the apoptotic peak reached on the 7th day after transplantation in transplant group. Apoptosis indices of liver cells in Emodin group were significantly lower than those in transplant group(Plt;0.01). Conclusion Emodin can inhibit hepatocellular apoptosis in rat liver transplantation. Key words Emodin； liver transplantation； graft rejection； apoptosis； rats 大黄素是中药大黄的有效成分，先前的实验研究表明，它能延长非同系大鼠原位肝移植的术后存活时间，能明显降低移植肝的排斥活动指数[1]。也有研究表明，移植肝排斥反应和细胞凋亡具有密切的相关性，抗排斥反应药物能有效地降低移植肝的细胞凋亡[2-3]。本实验采用近交系大鼠原位肝移植模型，探讨大黄素对肝移植急性排斥反应中肝细胞凋亡的影响。 1 材料和方法 1.1 药物和试剂 大黄素粉剂购自西安华萃公司，原位细胞凋亡检测(TUNEL检测)试剂盒及相关的染色试剂购自福建迈新公司。 1.2 实验动物和分组 实验大鼠购自北京动物实验中心。其中近交系Lewis(简称LEW)大鼠48只，近交系Brown Norway(简称BN)大鼠24只。质量220~280 g，均为雄性。将动物随机分为3组：?譹?訛对照组，供、受体各12只LEW大鼠；?譺?訛移植组，供、受体各12只，供体为LEW大鼠，受体为BN大鼠；?譻?訛大黄素组，在移植组基础上，肝脏移植术后每天以大黄素50 mg·kg-1腹腔注射。 1.3 指标检测和方法 　分别于移植术后第1，3