大黄酚对AD模型小鼠学习记忆影响.docVIP

大黄酚对AD模型小鼠学习记忆影响 　作者：董晓华 张海红 张丹参 张力 丁杰 【摘要】 目的 观察大黄酚对β淀粉样肽（Aβ2535）致AD小鼠学习记忆障碍及脑组织过氧化氢（H2O2）含量、过氧化氢酶（CAT）、Na+K+ATP酶和Ca2+ATP酶活性的影响。方法 给小鼠一次性脑室内注射凝聚态Aβ25353 μg（1.0 mmol/L）造AD模型，造模后腹腔注射药物或溶剂，连续注射14 d。采用Y迷宫法测试小鼠学习记忆能力，每天每鼠训练10次，连续测试5 d，以正确反应次数作为观测指标。用比色法测定小鼠脑组织H2O2含量、CAT、 Na+K+ATP酶和Ca2+ATP酶活性。结果 与对照组相比，模型组正确反应次数降低，脑内H2O2含量升高；CAT、Na+K+ATP酶和Ca2+ATP酶活性显著降低；与模型组比较，大黄酚各组小鼠正确反应次数从测试2 d起明显增加，小鼠脑内H2O2含量明显降低，而CAT、Na+K+ATP酶和Ca2+ATP酶活性显著升高。结论 大黄酚对Aβ2535致AD小鼠记忆障碍有保护作用，其作用机制可能与抗氧化、改善能量代谢有关。 【关键词】 大黄酚；β淀粉样肽；学习记忆；过氧化氢；过氧化氢酶；ATP酶 【Abstract】 Objective To observe the effects of chrysophanol on abilities of learning and memory, contents of H2O2, activities of catalase (CAT), Na+K+ATPase and Ca2+ATPase in brain tissue in AD model mice induced by Aβ2535. Methods The aggregated Aβ2535 3 μl (1 mmol/L) was intracerebroventricularly injected once into mice to make AD model. The solvent was administered by ip for control and model group, while chrysophanol (10.0,1.0,0.1 mg/kg) was injected for chrysophanol groups for 14 days. The abilities of learning and memory in mice were observed through Ymaze test for 5 days, in which every mouse was disciplined 10 times every day and the number of correct response was as observed indicators. Then the contents of H2O2 and the activities of CAT, Na+K+ATPase and Ca2+ATPase in brain tissue in mice were detected through colorimetry. Results Compared with control group, the number of correct response was decreased，the contents of H2O2 were increased, and activities of CAT, Na+K+ATPase and Ca2+ATPase were decreased significantly in model group. While the number was higher in chrysophanol group (10.0,1.0,0.1 mg/kg) from the second day, the contents of H2O2 were lower, and activities of CAT, Na+K+ATPase and Ca2+ATPase were higher than in model group significantly. Conclusions Chrysophanol shows protective effects on learning and memory impairment in AD mice induced by Aβ2535. Possible mechanisms are involved in the actions of anti