安乳颗粒中挥发油β-环糊精包合物制备工艺探究.docVIP

安乳颗粒中挥发油β-环糊精包合物制备工艺探究 　 作者：马趣环，石晓峰，范彬，沈薇 ，黄钰芳，张瑞堂 【摘要】 目的优选安乳颗粒中挥发油的提取及其β-环糊精的包合工艺。方法以提油量为指标，对影响挥发油提取的因素(加水量、提取时间)进行考察;以挥发油β-环糊精的包结率为考察指标，应用L9(34)正交实验法优选挥发油β-环糊精的包合工艺。结果挥发油的最佳提取工艺为加水8倍量，蒸馏6 h。影响挥发油包结因素大小依次为Agt;Cgt;B， 最佳工艺条件为：A2B3C2，即油∶β-CD配比为1∶6，恒温40℃搅拌包结1.5 h。结论该优选制备工艺合理，挥发油提出率和包结率高,适用于工业化生产。 【关键词】 安乳颗粒; 挥发油; 正交试验; 挥发油-β-环糊精包合物 　　Abstract：ObjectiveTo optimize the extraction procedure of volatile oil and preparation technique of β-CD inclusion complex of volatile oil in Anru granule.MethodsThe extraction volume of volatile oil was used as index, the influence factors (water volume and extraction time )on the yield of essential oil were studied. The inclusion ratio of β-CD inclusion complex of volatile oil was used as index, the optimum preparation conditions of β-CD inclusion complex by the orthogonal test of L9(34).ResultsThe optimal extraction procedure was that adding 8 times water and distillation 6h .The effects of inclusion were in the order of Agt; C gt; B ( A: volatile oil to β-CD ratio B: the inclusion temperature C: the inclusion time ),the optimal preparation conditions were A2B3C2, i.e. volatile oil to β-CD=1∶6, the inclusion temperature at 40℃ , the inclusion time of 1.5h .ConclusionThe optimum preparation procedure is reliable.The extraction volume of volatile oil and the inclusion ratio of β-CD inclusion complex of volatile oil are high .It can be applied to industrialized production. 　　Key words：Anru granule; Volatile oil; Orthogonal test; β-CD inclusion complex of volatile oil 　　安乳颗粒由柴胡、当归、赤芍、丹参等12味中药组成，具有软坚散结、理气止痛、活血消痛之效，对乳腺肿块、乳腺增生症有显著疗效。其处方中柴胡、当归、香附均含有挥发油，而挥发油是其重要的活性成分，因此，在安乳颗粒的制备工艺研究时，我们首先对这3种药材中挥发油的提取及其β-环糊精的包合工艺进行研究，希冀能最大限度的将挥发油提取完全，并经β-环糊精的包合，使成品安乳颗粒在贮存过程中减少氧化、变质和挥发损失。 　　1 仪器与试药 　　AE260万分之一分析天平(瑞士Mettle公司);旋转蒸发仪(瑞士BüCHI公司);PC-351 Laboratory hot plate stirrer(美国)。 　　β-环糊精[β-cyclodextrin(β-CD)，Q/CYDZ-622-98，中国医药集团上海化学试剂公司];水为超纯水，其余试剂均为分析纯。 　　药材柴胡、当归、香附购自甘肃兰州黄河药市，经甘肃省医学科学研究院分析试验中心检测均符合符合《中国药典》2005版Ⅰ部柴胡、当归、香附项下标准。 　　2 方法与结果 　　2.1 挥发油的提取 　　2.1.1