山茱萸多糖对衰老模型大鼠学习记忆能力影响探究.docVIP

山茱萸多糖对衰老模型大鼠学习记忆能力影响探究 　【摘要】 目的 以山茱萸多糖对D半乳糖致衰大鼠抗脑老化及学习记忆能力的影响，探讨其在改善脑老化方面的作用及可能机制。方法 采用D半乳糖诱导的衰老模型Wistar大鼠为实验对象，经胃灌服不同剂量山茱萸多糖(0.14、0.28 g/kg)，连续灌服30 d。测定各组大鼠学习记忆能力、脑组织丙二醛(MDA)、脂褐素(LF)含量、超氧化物歧化酶(SOD)和单胺氧化酶B(MAOB)活性，同时观察不同剂量山茱萸多糖对衰老模型大鼠上述指标的影响。结果 衰老模型组MDA、LF含量、MAOB活性较青年组明显增加(P＜0.01)，而学习记忆能力、SOD活性明显低于青年组(P＜0.01)；不同剂量山茱萸多糖组学习记忆能力、SOD活性明显高于衰老模型组(P＜0.05,P＜0.01)，而MDA、LF含量、MAOB活性显著低于衰老模型组(P＜0.05，P＜0.01)，以山茱萸多糖大剂量组效果更显著，山茱萸多糖大剂量组作用优于小剂量组。结论 山茱萸多糖可通过提高机体抗氧化能力、抑制脂质过氧化、提高老化相关酶活性发挥抗脑老化作用，提高大鼠学习记忆能力。 【关键词】 山茱萸多糖；衰老模型大鼠；脑老化；学习记忆能力 【Abstract】Objective To investigate the effects of dogwood polysaccharides on the antibrain aging and the capabilities of learning and memory in aging model rats induced by Dgalactose. Methods Senile model rats were induced by Dgalactose. The different doses of dogwood polysaccharides were intragastric administrated according to 0.14 and 0.28 g/kg wb for 30 d. The capabilities of learning and memory were measured, the content of malondialdehyde (MDA) and lipofuscin (LF), the activities of SOD and MAOB in brain tissue were tested. Results In senile model group, the contents of MDA, LF and the activity of MAOB were increased (P＜0.01) , the capabilities of learning and memory and the activity of SOD were decreased significantly (P＜0.01) than those in young group. Compared with those of senile model group, the capabilities of learning and memory and the activity of SOD were increased significantly (P＜0.05, P＜0.01) , while the contents of MDA, LF and the activity of MAOB were decreased (P＜0.05, P＜0.01) in two model dogwood polysaccharides groups. The effects of the large dose polysaccharide were more obvious than those of small dose. Conclusions Dogwood polysaccharides could enhance the antioxidation, the activity of SOD, inhibit lipid peroxidation, so it plays a certain role in antibrainaging and enhance the capabilities of learning and memory, especially in the large dose group. 　　【Key words】Dogwood polysaccharides; Aging model rats; Braina